2nd World Congress on
Primary Healthcare and Medicare Summit

Biography:

Abstract:

PURPOSE: To compare rates of positive bacterial growth in placental swab cultures done among women who received ampicillin prophylaxis at different timings after term PROM.


MATERIALS AND METHODS

Design: Matched Cohort Study

Setting: Department of Obstetrics and Gynecology at Southern Philippines Medical Center in Davao City, Philippines

Participants: One hundred twenty pregnant mothers at ≥37 weeks age of gestation, aged ≥18 years old who have a history of gross pooling of amniotic fluid and have a singleton pregnancy in vertex position and have no other infections and co-morbidities.

Methodology: A total of 120 patients consented and were included in the study.  These patients were grouped according to the onset of watery vaginal discharge up to the time where antibiotic was initiated.  They were then grouped in the 6^th hour, 12^th hour and 18^th hour group.  Blood tests such as WBC and CRP were obtained upon admission on these patients. Monitoring of maternal temperature and heart rate, fetal heart rate, monitoring for the occurrence of uterine tenderness and vaginal discharge were done.  After delivery, placental swab of the patients were submitted for culture and sensitivity.

 

Results: The mean age of patients belonging to the 6^th hour group is 25.4, 26 for patients in the 12^th hour group and 26.4 in the 18^th hour group.  Most of the patients were single with an OB Score of G1P0.  There were no significant differences in the age, civil status, gravidity and parity among the groups.  There were no significant differences in the clinical signs of intraamniotic infection (IAI) mentioned among the 3 groups.  There was no association between the occurrence of maternal infection based on WBC in the 3 groups.  However, more patients in the 12^th hour and 18^th hour  group have elevated WBC.  There is a significant association between CRP positivity and the 3 groups of PROM.  There are more patients in the 12^th hour group and 18^th hour group who showed a positive result for CRP.  For placental swab culture, there is no association between bacterial growth among the 3 groups.  Bacterial growth were observed in 27/40 (67.5%) of cultures in the 6H group, 31/40 (77.5%) of cultures in the 12H group, and 31/40 (77.5%) of cultures in the 18H group.  Across all groups, the five most common isolates were Escherichia coli, Staphylococcus hominis, Staphylococcus haemolyticus, Staphylococcus epidermidis, and Enterobacter cloacae.  In the 3 groups, the predominating bacteria was Escherichia coli.  This organism is 71% sensitive to Ampicillin and 100% sensitive to Amikacin, Amoxicillin/Clavulanic acid, Aztreonam, Cefepime, Cefotaxime, Cefoxitin, Cefuroxime, Ciprofloxacin, ertapenem, Imipenem   and Meropenem.

CONCLUSION: Among the 3 groups, there were no significant difference in their clinical signs of IAI.  However, laboratory parameters showed that there were more markers of infection seen on patients who received the antibiotic later.  Elevated WBC and bacterial growth in the placenta were higher in the 12^th hour and 18^th hour group, although not significantly different among the 3 groups.  There was also a significant difference in the CRP positivity among the 3 groups.  There were more patients who showed a positive CRP result on those who received the antibiotic later.  This study recommends that starting antibiotic earlier in the 6^th hour is beneficial.